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Recombinant Rat Resistin

Catalog #

5351

Price:

From $50.00 to $4,700.00
5ug - $50
25ug - $130
1000ug - $4,700

CERTIFICATE OF ANALYSIS AND DATA SHEET

5351: Recombinant Rat Resistin

PRODUCT DESCRIPTION:
Resistin, a product of the RSTN gene, is a peptide hormone belonging to the class of cysteine-rich secreted proteins (monomeric peptide contains 11 cysteine residues) referred to as the RELM family, and is also described as ADSF (Adipose Tissue-Specific Secretory Factor) or FIZZ3 (Found in Inflammatory Zone 3). Mouse resistin is expressed as a 114 amino acid prepeptide; its hydrofobic Nterminal 20 amino acid signal peptide is cleaved before its secretion. Mouse resistin circulates in blood as a homodimeric protein consisting of two 94 amino acid polypeptides, which are disulfide-linked via Cys26. Resistin may be an important link between obesity and insulin resistance. Mouse resistin, specifically produced and secreted by adipocyte, acts on skeletal muscle myocytes, hepatocytes and adipocytes themselves so that it reduces their sensitivity to insulin. Steppan et al. have suggested that resistin suppressed the ability of insulin to stimulate glucose uptake. They have also suggested that resistin was present at elevated levels in blood of obese mice, and was down regulated by fasting and by antidiabetic drugs. Way et al., on the other hand, have found that resistin expression is severely suppressed in obesity and is stimulated by several antidiabetic drugs. Other studies have shown that mouse resistin increases during the differentiation of adipocytes, but it also seems to inhibit adipogenesis. In contrast, the human adipogenic differentiation is likely to be associated with a down regulation of resistin gene expression.

The Recombinant Rat Resistin is manufactured with N-terminal fusion of His tag. The Recombinant Rat Resistin His-Tagged Fusion Protein is an 11.9 kDa protein containing 94 amino acid residues of the Rat Resistin and 16 additional amino acid residues.

SOURCE: Escherichia Coli.

FORMULATION:
Sterile filtered and lyophilized from 0.5 mg/ml in 20mM Tris pH 8.0

PURITY:
Greater than 95.0% as determined by: (a) Analysis by SDS-PAGE

STORAGE:
Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

SOLUBILITY: Add 0.2 ml of deionized H2O and let the lyophilized pellet dissolve completely.

 
DevaTal, Inc, 644 Newkirk Ave, Hamilton NJ 08610
Phone: 609-586-1575, Fax: 609-586-6892
FOR RESEARCH USE ONLY

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