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Recombinant rat leptin (rLEP) antagonist (triple mutant)

Catalog #

5347

Price:

From $100.00 to $2,300.00
20ug - $100
100ug - $250
1000ug - $2,300

CERTIFICATE OF ANALYSIS AND DATA SHEET

5347: Recombinant rat leptin (rLEP) antagonist (triple mutant)

PRODUCT DESCRIPTION:
Recombinant rat leptin, one polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kDa, rLEP was mutated, resulting in L39A/D40A/F41A mutant that was purified by proprietary chromatographic techniques.

SOURCE: Escherichia Coli.

FORMULATION:
The protein was lyophilized from a concentrated (0.85mg/ml) solution with 0.003mM NaHCO3.

PURITY:
Greater than 99.0% as determined by: (a) Gel filtration analysis. (b) Analysis by reducing and non-reducing SDS-PAGE gel.

STORAGE:
Lyophilized rLEP mutant although stable at room temperature for several weeks, should be stored desiccated below -18°C. Upon reconstitution at > 0.1 rLEP mutant mg/ml and up to 2 mM and filter sterilization rLEP mutant can be stored at 4°C or even room temperature for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles.

SOLUBILITY: It is recommended to reconstitute the lyophilized rLEP mutant in sterile water or sterile 0.4% NaHCO3 adjusted to pH 8-9, not less than 100ug/ml, which can then be further diluted with other aqueous solutions.

BIOLOGICAL ACTIVITY: rLEP triple antagonist is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. It also inhibits various leptin effects in several in vitro bioassays.

 
DevaTal, Inc, 644 Newkirk Ave, Hamilton NJ 08610
Phone: 609-586-1575, Fax: 609-586-6892
FOR RESEARCH USE ONLY
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. (c) Anion-exchange FPLC

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