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Recombinant Rat Leptin (Anti-Obesity Protein)

Catalog #

5348

Price:

From $50.00 to $450.00
200ug - $50
1000ug - $130
5000ug - $450

CERTIFICATE OF ANALYSIS AND DATA SHEET

5348: Recombinant Rat Leptin (Anti-Obesity Protein)

PRODUCT DESCRIPTION:
Recombinant Rat Leptin produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 147 amino acids and having a molecular mass of 16,240 Dalton. The rRLeptin is purified by proprietary chromatographic techniques.

SOURCE: Escherichia Coli.

FORMULATION:
The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3.

PURITY:
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. (c) Anion-exchange FPLC

STORAGE:
Although stable at room temperature for 3 weeks, it should be stored desiccated below -18°C. Upon reconstitution store at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

SOLUBILITY: It is recommended to reconstitute in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

BIOLOGICAL ACTIVITY: rRLeptin is fully biologically active when compared to standards. The ED50, calculated by the leptin-dependant stimulation of Human OB-R transfected murine BaF3 indicator cells is 0.35-0.06 ng/ml.

 
DevaTal, Inc, 644 Newkirk Ave, Hamilton NJ 08610
Phone: 609-586-1575, Fax: 609-586-6892
FOR RESEARCH USE ONLY

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