Menu

Recombinant Rat Interleukin-18

Catalog #

5346

Price:

From $50.00 to $5,000.00
5ug - $50
20ug - $130
1000ug - $5,000

CERTIFICATE OF ANALYSIS AND DATA SHEET

5346: Recombinant Rat Interleukin-18

PRODUCT DESCRIPTION:
Recombinant Rat IL-18 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 158 amino acids and having a molecular mass of 18260 Dalton. The rrIL-18 is purified by proprietary chromatographic techniques.

SOURCE: Escherichia Coli.

FORMULATION:
Lyophilized from a concentrated (1mg/ml) solution in water containing 0.4 x PBS pH=7.2

PURITY:
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. (c) Anion-exchange FPLC

STORAGE:
Although stable at room temperature for 3 weeks, it should be stored desiccated below -18°C. Upon reconstitution store at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

SOLUBILITY: It is recommended to reconstitute in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

BIOLOGICAL ACTIVITY: rrIL-18 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of IFN-gamma by murine lymph node cells (4 x 105 cells/well) co-stimulated with sub optimal dose of ConA (0.75ug/ml) is 12 ng/ml. The medium was RPMI containing 10% FCS and 50uM 2-ME.

 
DevaTal, Inc, 644 Newkirk Ave, Hamilton NJ 08610
Phone: 609-586-1575, Fax: 609-586-6892
FOR RESEARCH USE ONLY

Share this product