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Recombinant Rat Interleukin-1 beta

Catalog #

5336

Price:

From $50.00 to $5,700.00
2ug - $50
10ug - $130
1000ug - $5,700

CERTIFICATE OF ANALYSIS AND DATA SHEET

5336: Recombinant Rat Interleukin-1 beta

PRODUCT DESCRIPTION:
Interleukin-1b is produced by activated macrophages, IL-1B stimulates thymocyte proliferation by inducing il-2 release, b-cell maturation and proliferation, and fibroblast growth factor activity. IL1B proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.

Interleukin-1b Rat Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 153 amino acids and having a molecular mass of 17415.82 Dalton. The IL-1b is purified by proprietary chromatographic techniques.

SOURCE: Escherichia Coli.

FORMULATION:
The protein was lyophilized without any additives.

PURITY:
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

STORAGE:
Although stable at room temperature for 3 weeks, it should be stored desiccated below -18°C. Upon reconstitution store at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

SOLUBILITY: It is recommended to reconstitute in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

BIOLOGICAL ACTIVITY: The ED50 range= 0.2-0.5 ng/ml, determined by the dose dependent proliferation of mouse D10S cells.

 
DevaTal, Inc, 644 Newkirk Ave, Hamilton NJ 08610
Phone: 609-586-1575, Fax: 609-586-6892
FOR RESEARCH USE ONLY

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