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Recombinant Rat Clusterin (Apoliprotein J)

Catalog #

5327

Price:

From $50.00 to $4,000.00
2ug - $50
10ug - $130
1000ug - $4,000

CERTIFICATE OF ANALYSIS AND DATA SHEET

5327: Recombinant Rat Clusterin (Apoliprotein J)

PRODUCT DESCRIPTION:
Clusterin also named Apoliprotein J is a 75-80 kD disulfide-linked heterodimeric protein containing about 30% of N-linked carbohydrate rich in sialic acid but truncated forms targeted to the nucleus have also been identified. The precursor polypeptide chain is cleaved proteolytically to remove the 22-mer secretory signal peptide and subsequently between residues 227/228 to generate the  and  chains. These are assembled in anti-parallel to give a heterodimeric molecule in which the cysteine-rich centers are linked by five disulfide bridges and are flanked by two predicted coiled-coil -helices and three predicted amphipathic a-helices. Across a broad range of species clusterin shows a high degree of sequence homology ranging from 70% to 80%. It is nearly ubiqitously expressed in most mammalian tissues and can be found in plasma, milk, urine, cerebrospinal fluid and semen. It is able to bind and form complexes with numerous partners such as immunoglobulins, lipids, heparin, bacteria, complement components, paraoxonase, beta amyloid, leptin and others. Clusterin has been ascribed a plethora of functions such as phagocyte recruitment, aggregation induction, complement attack prevention, apoptosis inhibition, membrane remodelling, lipid transport, hormone transport and/or scavenging, matrix metalloproteinase inhibition. A genuine function of clusterin has not been defined. One tempting hypothesis says that clusterin is an extracellular chaperone protecting cells from stress induced insults caused by degraded and misfolded protein precipitates. Clusterin is up- or downregulated on the mRNA or protein level in many pathological and clinically relevant situations including cancer, organ regeneration, infection, Alzheimer disease, retinitis pigmentosa, myocardial infarction, renal tubular damage, autoimmunityand others.

The Rat Clusterin was constructed as a recombinant protein with N-terminal fusion of T7-Tag (16AA) and C-terminal fusion of His-Tag (9AA). The Rat Clusterin His-Tagged Fusion Protein, produced in E.coli, is 26.5 kDa protein containing 215 amino acid residues of the Rat Clusterin and 25 additional amino acid residues.

SOURCE: Escherichia Coli.

FORMULATION:
Sterile filtered and lyophilized from 0.5 mg/ml in 0.01M Tris pH 7.2

PURITY:
Greater than 95.0%

STORAGE:
Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

SOLUBILITY: Add 0.2 ml of deionized H2O and let the lyophilized pellet dissolve completely.

 
DevaTal, Inc, 644 Newkirk Ave, Hamilton NJ 08610
Phone: 609-586-1575, Fax: 609-586-6892
FOR RESEARCH USE ONLY

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