Menu

Recombinant Human Fibroblast Growth Factor-basic Sf9 Insect Cells derived (rHuFGF-b-Sf9)

Catalog #

5042

Price:

From $50.00 to $6,570.00
2ug - $50
10ug - $130
1000ug - $6,570

CERTIFICATE OF ANALYSIS AND DATA SHEET

PRODUCT DESCRIPTION:
Recombinant Human FGF-b (FGF-2) produced in Sf9 insect cells is a single, glycosylated, polypeptide chain containing 155 amino acids and having a molecular mass of 17353 Dalton. The rHuFGF-b is purified by proprietary chromatographic techniques.

SOURCE: Baculovirus.

FORMULATION:
The sterile protein solution (0.5mg/ml) contains 20mM Tris pH=7.9, 100mM KCl, 1mM DTT and 20% glycerol.

PURITY:
Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by SDS-PAGE

STORAGE:
Although stable at room temperature for 3 weeks, it should be stored desiccated below -18°C. Upon reconstitution it should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.

BIOLOGICAL ACTIVITY: rHuFGF-b is fully biologically active when compared to standards. The ED50, calculated by the dose-dependant proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake) is <0.5 ng/ml, corresponding to a specific activity of 2 x 106 Units/mg.

ENDOTOXIN: Less than 0.1 ng/ug (IEU/ug) of rHuFGF-b.

 
DevaTal, Inc, 644 Newkirk Ave, Hamilton NJ 08610
Phone: 609-586-1575, Fax: 609-586-6892
FOR RESEARCH USE ONLY

Share this product