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Recombinant Human Fibroblast Growth Factor-acidic (rHuFGF-a)

Catalog #

7031

Price:

From $60.00 to $1,200.00
10ug - $60
50ug - $150
1000ug - $1,200

CERTIFICATE OF ANALYSIS AND DATA SHEET

7031: Recombinant Human Fibroblast Growth Factor-acidic (rHuFGF-a)

PRODUCT DESCRIPTION:
A well-characterized basic peptide believed to be secreted by the liver and to circulate in the blood. It has growth-regulating, insulin-like, and mitogenic activities. This growth factor has a major, but not absolute, dependence on Somatotropin. It is believed to be mainly active in adults in contrast to Insulin like Growth Factor 2, which is a major fetal growth factor. Recombinant murine IGF-1 produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7600 Dalton.

SOURCE:
E.coli.

FORMULATION:
Recombinant Murine IGF-1 was lyophilized after extensive dialysis against 0.2ᄉm-filtered solution in PBS, pH 7.2.

RECONSTITUTION:
It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mgs/ml, which can be further diluted into other aqueous solutions.

STABILITY:
Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.

PURITY:
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Reducing and non-reducing SDS-PAGE.

PROTEIN CONTENT DETERMINED BY:
UV spectroscopy at 280 nm.
Analysis by RP-HPLC calibrated against a known standard.
Quantitation on SDS-PAGE against a known standard.

BIOLOGICAL ACTIVITY:
IGF-I is fully biologically active when compared to standards. The ED50, calculated by the dose-dependant proliferation of murine BALBC 3T3 cells (measured by 3H-thymidine uptake) is less then 1.0 ng/ml, corresponding to a specific activity of 1x10 U/mg.

 
DevaTal, Inc, 644 Newkirk Ave, Hamilton NJ 08610
Phone: 609-586-1575, Fax: 609-586-6892
FOR RESEARCH USE ONLY

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