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Recombinant Human Fibroblast Growth Factor-acidic (rHuFGF-a)

Catalog #

5041

Price:

From $50.00 to $1,350.00
10ug - $50
50ug - $130
1000ug - $1,350

CERTIFICATE OF ANALYSIS AND DATA SHEET

PRODUCT DESCRIPTION:
Recombinant Human FGF-a (FGF-1) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 140 amino acids and having a molecular mass of 15803 Dalton. The rHuFGF-a is purified by proprietary chromatographic techniques.

SOURCE: Escherichia Coli.

FORMULATION:
The protein was lyophilized from a concentrated (1mg/ml) sterile solution containing 10mM Tris pH=7.6 and 100mM NaCl.

PURITY:
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by SDS-PAGE

STORAGE:
Although stable at room temperature for 3 weeks, it should be stored desiccated below -18°C. Upon reconstitution it should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.

SOLUBILITY:
It is recommended to reconstitute in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

BIOLOGICAL ACTIVITY: rHuFGF-a is fully biologically active when compared to standards. The ED50, calculated by the dose-dependant proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake) is <10 ng="" ml="" corresponding="" to="" a="" specific="" activity="" of="" 105="" units="" mg="" p="">

ENDOTOXIN: Less than 0.1 ng/ug (IEU/ug) of rHuFGF-a.

 
DevaTal, Inc, 644 Newkirk Ave, Hamilton NJ 08610
Phone: 609-586-1575, Fax: 609-586-6892
FOR RESEARCH USE ONLY

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