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Recombinant Human Epidermal Growth Factor (rHuEGF)

Catalog #

5086

Price:

From $50.00 to $230.00
100ug - $50
500ug - $130
1000ug - $230

CERTIFICATE OF ANALYSIS AND DATA SHEET

PRODUCT DESCRIPTION:
Recombinant Human EGF produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 53 amino acids and having a molecular mass of 6222 Dalton. The rHuEGF is purified by proprietary chromatographic techniques.

SOURCE: Escherichia Coli.

FORMULATION:
The protein was lyophilized from a concentrated (1mg/ml) solution with no additives.

PURITY:
Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE (c) Anion-exchange FPLC

STORAGE:
Although stable at room temperature for 3 weeks, it should be stored desiccated below -18°C. Upon reconstitution it should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.

SOLUBILITY:
It is recommended to reconstitute in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

BIOLOGICAL ACTIVITY: rHuEGF is fully biologically active when compared to standards. The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells (measured by 3H-thymidine uptake) is < 0.1 ng/ml corresponding to a specific activity of 1 x 107 Units/mg.

ENDOTOXIN: Less than 0.1 ng/ug (IEU/ug.

 
DevaTal, Inc, 644 Newkirk Ave, Hamilton NJ 08610
Phone: 609-586-1575, Fax: 609-586-6892
FOR RESEARCH USE ONLY

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