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Recombinant Human Connective Tissue Growth Factor (rHuCTGF)

Catalog #

7032

Price:

From $60.00 to $3,200.00
2ug - $60
10ug - $150
1000ug - $3,200

CERTIFICATE OF ANALYSIS AND DATA SHEET

7032: Recombinant Human Connective Tissue Growth Factor (rHuCTGF)

PRODUCT DESCRIPTION:
CTGF belongs to the CCN family of secreted proteins. CTGF is a member of cysteine rich regulatory proteins playing an important role in mitogenic and chemotaxis. Each protein has an IGF-binding domain, a thrombospoindin type 1 domain and cysteine knot region. CTGF has multiple effects on development and differentiation. H-CTGF is comprised of 98 amino acids with a molecular weight of 11.2 kDa.

SOURCE:
E.coli.

FORMULATION:
Sterile filtered and lyophilized from 1.0 mg/ml in 10 mM NaAcetate buffer pH 6.0.

RECONSTITUTION:
Reconstitute at 0.1 mg/ml with 5 mM NaAcetate, 6.0.

STABILITY:
Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.

PURITY:
Greater than 95% as determined by:
Analysis by RP-HPLC.
Reducing and non-reducing SDS-PAGE.

PROTEIN CONTENT DETERMINED BY:
UV spectroscopy at 280 nm.
Analysis by RP-HPLC calibrated against a known standard.
Quantitation on SDS-PAGE against a known standard.

BIOLOGICAL ACTIVITY:
Determined by the dose-dependent stimulation of the proliferation of HUVEC cells. The expected ED50 for this effect is 1.0-2.0 ug/ml.

 
DevaTal, Inc, 644 Newkirk Ave, Hamilton NJ 08610
Phone: 609-586-1575, Fax: 609-586-6892
FOR RESEARCH USE ONLY

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