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Recombinant Human Ciliary Neurotrophic Facor (rHuCNTF)

Catalog #

5079

Price:

From $50.00 to $2,715.00
5ug - $50
20ug - $130
1000ug - $2,715

CERTIFICATE OF ANALYSIS AND DATA SHEET

PRODUCT DESCRIPTION:
Recombinant Human CNTF produced in E.Coli is a single, non-glycosylated polypeptide chain containing 199 amino acids and having a molecular mass of 22706 Dalton. The rHuCNTF is purified by proprietary chromatographic techniques.

SOURCE: Escherichia Coli.

FORMULATION:
Lyophilized from a concentrated (1mg/ml) solution in water containing 5mM sodium Phosphate buffer pH=7.5 and 5mM sodium chloride.

PURITY:
Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE (c) Anion-exchange FPLC

STORAGE:
Although stable at room temperature for 3 weeks, it should be stored desiccated below -18°C. Upon reconstitution it should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.

SOLUBILITY:
It is recommended to reconstitute in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

BIOLOGICAL ACTIVITY: rHuCNTF is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of TF-1 cells is < 2 ng/ml, corresponding to a Specific Activity of 5 x 105 IU/mg.

ENDOTOXIN: Less than 0.1 ng/ug (IEU/ug.

 
DevaTal, Inc, 644 Newkirk Ave, Hamilton NJ 08610
Phone: 609-586-1575, Fax: 609-586-6892
FOR RESEARCH USE ONLY

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