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Recombinant Human Brain-derived Neurotrophic Factor (rHuBDNF)

Catalog #

7027

Price:

From $60.00 to $4,800.00
2ug - $60
10ug - $150
1000ug - $4,800

CERTIFICATE OF ANALYSIS AND DATA SHEET

7027: Recombinant Human Brain-derived Neurotrophic Factor (rHuBDNF)

PRODUCT DESCRIPTION:
BDNF is a member of the nerve growth factor family of trophic factors. In the brain BDNF has a trophic action on retinal, cholinergic, and dopaminergic neurons, and in the peripheral nervous system it acts on both motor and sensory neurons. Some protein domains of BDNF are identical with those of NGF and another neurotrophic factor, designated NT-3 (neurotrophin-3 ).Polyclonal antibodies raised against murine NGF have been shown to cross-react with both NT-3 and BDNF. Recombinant Human Brain-Derived Neurotrophic Factor produced in E.Coli is a homodimer , non-glycosylated, polypeptide chain containing 2 x 119 amino acids and having a total molecular mass of 26,984 Dalton.

SOURCE:
E.coli.

FORMULATION:
Lyopohilized with no additives.

RECONSTITUTION:
It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mgs/ml, which can be further diluted into other aqueous solutions.

STABILITY:
Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.

BIOLOGICAL ACTIVITY:
BDNF is fully biologically active when compared to standard. The ED50, calculated by the dose-dependant induction of ACHE (acetylcholine esterase) in rat basal forebrain primary septal culture is 50 ng/ml.

PURITY:
Greater than 96.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Reducing and non-reducing SDS-PAGE.

PROTEIN CONTENT DETERMINED BY:
UV spectroscopy at 280 nm.
Analysis by RP-HPLC calibrated against a known standard.
Quantitation on SDS-PAGE against a known standard.

 
DevaTal, Inc, 644 Newkirk Ave, Hamilton NJ 08610
Phone: 609-586-1575, Fax: 609-586-6892
FOR RESEARCH USE ONLY

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