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Recombinant Human beta-Nerve Growth Factor (rHub-NGF)

Catalog #

5066

Price:

From $50.00 to $2,715.00
5ug - $50
20ug - $120
1000ug - $2,715

CERTIFICATE OF ANALYSIS AND DATA SHEET

PRODUCT DESCRIPTION:
Recombinant Human -NGF produced in CHO is a homodimer, glycosylated, polypeptide chain containing 2 identical 119 amino acids and having a molecular mass of 16,950Dalton. The rHu-NGF is purified by proprietary chromatographic techniques.

SOURCE: CHO Cells

FORMULATION:
The protein was lyophilized from a 0.2um filtered solution in 30mM sodium acetate pH=5.5 containing 1% HSA

PURITY:
Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE (c) Anion-exchange FPLC

STORAGE:
Although stable at room temperature for 3 weeks, it should be stored desiccated below -18°C. Upon reconstitution it should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.

SOLUBILITY:
It is recommended to reconstitute in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

BIOLOGICAL ACTIVITY: rHub-NGF is fully biologically active when compared to standards. The ED50, calculated by its ability to stimulate chick E9 DRG neurite outgrowth was found to be < 1.0 ng/ml, corresponding to a specific activity of > 1 x 106 units/mg..

ENDOTOXIN: Less than 0.1 ng/ug (IEU/ug)

 
DevaTal, Inc, 644 Newkirk Ave, Hamilton NJ 08610
Phone: 609-586-1575, Fax: 609-586-6892
FOR RESEARCH USE ONLY

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