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Recombinant Human Angiopoietin-like Protein 4 (rHuANGPTL4)

Catalog #

5059

Price:

From $50.00 to $5,430.00
2ug - $50
10ug - $130
1000ug - $5,430

CERTIFICATE OF ANALYSIS AND DATA SHEET

PRODUCT DESCRIPTION:
The fasting-induced adipose factor (FIAF, ANGPTL4, PGAR, HFARP) was identified as an adipocytokine up-regulated by fasting, by peroxisome proliferator-activated receptor agonists, and by hypoxia. At the protein level, in human and mouse blood plasma, FIAF was found to be present both as a native protein and in a truncated form. Differentiation of mouse 3T3-L1 adipocytes was associated with the production of truncated FIAF, whereas in human white adipose tissue and SGBS adipocytes, only the native FIAF could be detected. Interestingly, the truncated FIAF was produced by human liver. Experimental data suggest that FIAF is mainly presented in human blood plasma in a truncated form (FIAF-S2), whose level is increased by fenofibrate treatment. Levels of both truncated and native FIAF showed marked inter individual variation but were not associated with body mass index and were not influenced by prolonged semistarvation. The Recombinant Human ANGPTL4 is manufactured with N-terminal fusion of HisTag. The rHuANGPTL4 His -Tagged Fusion Protein is 25 kDa protein containing 204 amino acid residues of the Human ANGPTL4 and 16 additional amino acid residues.

SOURCE: E.coli

FORMULATION:
Sterile filtered and lyophilized from 0.5 mg/ml in 0.05M Acetate buffer pH 4.

PURITY:
Greater than 95.0%

STORAGE:
Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

SOLUBILITY:
Add 0.2 ml of 0.1M Acetate buffer pH4 and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10ug/ml. In higher concentrations the solubility of this antigen is limited

 
DevaTal, Inc, 644 Newkirk Ave, Hamilton NJ 08610
Phone: 609-586-1575, Fax: 609-586-6892
FOR RESEARCH USE ONLY

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