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Recombinant Human Adiponectin, His tag

Catalog #

5033

Price:

From $50.00 to $2,000.00
10ug - $50
50ug - $130
1000ug - $2,000

CERTIFICATE OF ANALYSIS AND DATA SHEET

PRODUCT DESCRIPTION:
Adiponectin, also referred to as Acrp30, AdipoQ and GBP-28, is a recently discovered 244 amino acid protein, the product of the apM1 gene, which is physiologically active and specifically and highly expressed in adipose cells (Adipokine). The protein belongs to the soluble defense collagen super family; it has a collagen-like domain structurally homologous with collagen VIII and X and complement factor C1q-like globular domain. APM-1 forms homotrimers, which are the building blocks for higher order complexes found circulating in serum. The Adiponectin Human is created as a recombinant protein with N-terminal fusion of His Tag. The Adiponectin His-Tagged Fusion Protein, produced in E. coli, is 26.4 kDa protein containing 230 amino acid residues of the Acrp30 human and 12 additional amino acid residues.

SPECIFICITY: The amino acid sequence of the APM-1 Human Recombinant is 100% homologous to the amino acid sequence of the Adiponectin human serum.

PURIFICATION METHOD: Three-step procedure using affinity Ni-NTA chromatography and size exclusion chromatography before and after refolding.

SOURCE:
Escherichia Coli.

PURITY:
Greater than 95% as determined by SDS-PAGE analyzed.

FORMULATION:
Sterile filtered and lyophilized from 0.5 mg/ml in 0.02M Tris buffer pH7.5, 0.15M NaCl.

SOLUBILITY:
Add 0.2 ml of 0.1M Acetate buffer pH4 and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10ug/ml. In higher concentrations the solubility of this antigen is limited.

STABILITY: Store lyophilized Adiponectin at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. The lyophilized APM-1 remains stable until the expiry date when stored at -20°C.

 
DevaTal, Inc, 644 Newkirk Ave, Hamilton NJ 08610
Phone: 609-586-1575, Fax: 609-586-6892
FOR RESEARCH USE ONLY

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