Mouse I-Ak Mouse IgG

CLONE #: IA-G3

IMMUNOGEN:
Purified Mouse lymph node B cells.

Ig SUBCLASS:
IgG 2 Mouse

PURIFICATION METHOD:
Protein A column

PROTEIN CONCENTRATION:
1 mg/ml in PBS (after reconstitution).

STORAGE:
Lyophilized: store at 4°C. After reconstitution, if not used within a month, aliquot and store at -20°C.

STABILITY / SHELF LIFE:
Two years lyophilized. One month in solution at 4°C.

RECONSTITUTION / USAGE
Reconstitute with 0.2 ml of H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. For flow cytometry, add 5-10 ul per 106 cells.

SPECIFICITY:
I-Ak

CROSSREACTIVITY:
No crossreactivity with other major haplotypes was detected.

APPLICATIONS:
Staining, sorting, panning, immunoprecipitation, and antibody mediated cytotoxicity.