Mouse I-Ab,d Mouse IgG

CLONE #: IA-C6

CATALOG #: 2003: Purified, Biotin, or FITC

IMMUNOGEN:
Purified Mouse lymph node B cells.

Ig SUBCLASS:
IgM, Mouse

PURIFICATION METHOD:
Boric Acid Precipitation / S-Sepharose.

PROTEIN CONCENTRATION:
1 mg/ml in PBS (after reconstitution).

STORAGE PROCEDURE:
Lyophilized: store at 4°C. After reconstitution, if not used within a month, aliquot and store at -20°C.

STABILITY / SHELF LIFE:
Two years lyophilized, one month in solution at 4°C.

RECONSTITUTION / USAGE:
Reconstitute with 0.2 ml of H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. For flow cytometry, add 5-10 ul per 106 cells.

SPECIFICITY:
Mouse I-Ab,d

CROSSREACTIVITY:
No crossreactivity detected with other major haplotypes.

APPLICATIONS:
Staining, sorting, panning, antibody mediated cytotoxicity and immunoprecipitation.