Human MIP-1 alpha, Mouse IgG

CLONE #: mxsghk-mcaf

CATALOG #: 1718

IMMUNOGEN:
r.Human MCAF

Ig SUBCLASS:
Mouse IgG2a

PURIFICATION METHOD:
Protein A / Ssepharose.

PROTEIN CONCENTRATION:
1 mg/ml in PBS (after reconstitution)

STORAGE PROCEDURES:
Lyophilized: store at 4°C. After reconstitution, if not used within a month, aliquot and store at 20°C.

STABILITY / SHELF LIFE:
Two years lyophilized, one month in solution at 4°C.

RECONSTITUTION:
Reconstitute with 0.2 ml of H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.

TITER
In direct ELISA, using alkaline phosphatase goat anti-mouse Ig (Jackson Laboratories) 1:5,000 dilution will give 0.3 O.D within 10 minutes.

NOTE: MCAF does not bind to plastic as well as most proteins – use higher concentration for proper binding.