Description:
Recombinant Rat MCP-1 produced in E.Coli is a non-glycosylated, Polypeptide chain containing 125 amino acids and having a molecular mass of 14092 Dalton. The rRMCP-1 is purified by proprietary chromatographic techniques.

Source:
Escherichia Coli.

Formulation:
The protein was lyophilized from a concentrated (1mg/ml) sterile solution containing no additives.

Solubility:
It is recommended to reconstitute the lyophilized rRMCP-1 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability:
Lyophilized rRMCP-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution rRMCP-1 should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Purity:
Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Amino acid sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Gln-Pro-Asp-Ala-Val.

Dimers and aggregates:
Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity:
Fully biologically active when compared to standard. The specific activity as determined by the ability of Rat MCP-1 to chemoattract human Monocytes at 10-100ng/ml.

Endotoxin:
Less than 0.1 ng/µg (IEU/µg) of RMCP-1.

Protein content:
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm.
2. Analysis by RP-HPLC, using a standard solution of Rat MCP-1 as a Reference Standard.