PRODUCT DESCRIPTION:
Interleukin-6 is a potent pro-inflammatory cytokine primarily produced by activated T cells and an assortment of other cells including endothelial cells and macrophages. IL-6 affects B and T lymphocytes and has been shown to have a role in host defense, acute phase reactions, immune responses and hematopoiesis.

SOURCE:
Escherichia Coli.

FORMULATION:
The protein was lyophilized without any additives.

PURITY:
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

STORAGE:
Although stable at room temperature for 3 weeks, it should be stored desiccated below -18°C. Upon reconstitution store at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

SOLUBILITY:
It is recommended to reconstitute the lyophilized Interleukin-6 in 100mM acetic acid to 1.0 mg/mL and incubated for 30 minutes at room temperature to regain full activity, which can then be further diluted to other aqueous solutions.

BIOLOGICAL ACTIVITY:
The ED50= 0.03-0.1 ng/mL. The biological activity is determined by measuring the dose-dependant proliferation of IL-6 dapendent B9 cells. A concentration range of 0.1 to 10.0 ng/mL is effective for most in vitro applications.