PRODUCT DESCRIPTION:
Recombinant Rat IL-12 produced in Sf9 insect cells is a glycosylated disulfide linked heterodimeric polypeptide containing 503 amino acids and having a molecular weight of 70 kDa comprised of disulfide-bonded 35 kDa (p35) and 40 kDa (p40) subunits. The rrIL-12 is purified by proprietary chromatographic techniques.

SOURCE:
Baculovirus

FORMULATION:
The protein was lyophilized without any additives.

PURITY:
Greater than 95.8% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(c) Anion-exchange FPLC

STORAGE:
Although stable at room temperature for 3 weeks, it should be stored desiccated below -18°C. Upon reconstitution store at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

SOLUBILITY:
It is recommended to reconstitute in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

BIOLOGICAL ACTIVITY:
rrIL-12 is fully biologically active when compared to standard. The ED50 was determined by the stimulation of IFN-gamma production by murine splenocyes co-stimulated with IL-12 and was found to be < 0.1ng/ml, corresponding to a specific activity of >1 x 107.