PRODUCT DESCRIPTION:
Recombinant Murine IL-7 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 130 amino acids and having a molecular mass of 14897 Dalton. The rmIL-7 is purified by proprietary chromatographic techniques.

SOURCE:
Escherichia Coli.

FORMULATION:
The protein was lyophilized from a concentrated (1mg/ml) solution containg 50 mM NH4HCO3 , pH 8.0.

PURITY:
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE
(c) Anion-exchange FPLC

STORAGE:
Although stable at room temperature for 3 weeks, it should be stored desiccated below -18°C. Upon reconstitution store at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

SOLUBILITY:

BIOLOGICAL ACTIVITY:
rmIL-7 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of murine 2E8 cells is < 0.2ng/ml, corresponding to a Specific Activity of 2 x 106 IU/mg.