Description:
Recombinant Mouse KC also nown as N51 and GRO-1 produced in E.Coli is a single,non-glycosylated, polypeptide chain containing 72 amino acids and having a molecular mass of 7815 Dalton. Purified by proprietary chromatographic techniques.

Source:
Escherichia Coli.

Formulation:
The protein was lyophilized with no additives.

Solubility:
It is recommended to reconstitute the lyophilized rmKC in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability:
Lyophilized rmKC although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution rmKC should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Purity:
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.

Amino acid sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Ile-Ala-Asn.

Dimers and aggregates:
Less than 1% as determined by silver stained SDS-PAGE gel analysis.

Biological Activity:
The biological activity was determined by measuring the dose dependent mobilization of intracellular calcium (calcium flux) with human neutrophils. Significant calcium mobilization is observed with ≥50ng/mL of recombinant mouse KC. The optimal concentration for each specific application should be determined by an initial dose-response assay.

Endotoxin:
Less than 0.1 ng/µg (IEU/µg) of rmKC

Protein content:
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.03 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of KC as a Reference Standard.