|
Price: From $50.00 to $6,400.00
Product Options
| |
|
|
CERTIFICATE OF ANALYSIS AND DATA SHEET |
|
PRODUCT DESCRIPTION:
Adiponectin (also called ACRP30 and GBP28) is a protein exclusively secreted from adipose tissue.
SPECIFICITY:
The recombinant mouse adiponectin is 100% homologous with the Mouse serum adiponectin.
SOURCE:
HEK293 (Human embryonic kidney cell line)
PURITY:
Purity of recombinant mouse adiponectin is >98% (HPLC and SDS PAGE analyzed).
ACTIVITY IN VITRO:
Full-length adiponectin has been shown to activate AMP-activated protein
kinase in hepatocyte (Yamauchi T. et al., 2002). It can also activate AMPK in HepG2 human hepatocytes at the concentration of as low as 1.0 ug/ml.
ACTIVITY IN VIVO:
Full-length adiponectin purified from mammalian cells can acutely decrease blood glucose levels (Berg A.H. et al., 2001). Single subcutaneous injection of adiponectin at the dose of 30 mg/g body weight significantly decreased the blood glucose levels in C57 mice. In vitro gluconeogenesis assay in primary rat hepatocytes was performed, showing the murine adiponectin derived from mammalian cells can inhibit glucose production. Effect of murine adiponectin on gluconeogenesis in primary rat hepatocytes. Primary rat hepatocytes were isolated and maintained as we described previously (Wang et al.,2002). The cells were then treated with 50 pM insulin plus different concentration of murine adiponectin purified from HEK293 cells for 48 hours. At the end of treatment, the cells were incubated with a glucose-free medium for 6 hours, and glucose production was measured as we described previously (n=4-6).
FORMULATION:
Sterile filtered and lyophilized from 0.5 mg/ml in TBS buffer.
RECONSTITUTION:
Add 0.2 ml of deionized water and let the lyophilized pellet dissolve completely.
STORAGE:
Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
STABILITY AND SHELF LIFE:
The lyophilized protein remains stable until the expiry date when stored at -20°C
|
| |
DevaTal, Inc, 644 Newkirk Ave, Hamilton NJ 08610
Phone: 609-586-1575, Fax: 609-586-6892 |
FOR RESEARCH USE ONLY |
|
|
|
|