Description:
Recombinant Human GRO-beta produced in E.Coli is a single,non-glycosylated, polypeptide chain containing 73 amino acids and having a molecular mass of 7908 Dalton. Purified by proprietary chromatographic techniques.

Source:
Escherichia Coli.

Formulation:
The protein was lyophilized with no additives.

Solubility:
It is recommended to reconstitute the lyophilized rHuGRO-beta in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability:
Lyophilized rHuGRO-beta although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution rHuGRO-beta should be stored at 4C between 2-7 days and for future use below -18C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Purity:
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.

Amino acid sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Leu-Ala-Thr.

Dimers and aggregates:
Less than 1% as determined by silver stained SDS-PAGE gel analysis.

Biological Activity:
rHuGRO-beta is fully biologically active when compared to standard.
The Biological activity is calculated by its ability to chemoattract CXCR2 transfected 293 cells using 10.0-100.0 ng/ml.

Endotoxin:
Less than 0.1 ng/µg (IEU/µg) of rHuGRO-beta.

Protein content:
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm.
2. Analysis by RP-HPLC, using a standard solution of GRO-beta as a Reference Standard.