Catalog #:
4102

Description:
Recombinant Human ENA-78 produced in E.Coli is a single, non-glycosylated,
polypeptide chain containing 74 amino acids and having a molecular mass of 8020 Dalton. Purified by proprietary chromatographic techniques.

Source:
Escherichia Coli.

Formulation:
Lyophilized from a concentrated (1mg/ml) solution in water containing no additives.

Solubility:
It is recommended to reconstitute the lyophilized ENA-78 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability:
Lyophilized ENA-78 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution ENA-78 should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Purity:
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Amino acid sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be, Ala- Ala -Val-Leu-Arg.

Dimers and aggregates:
Less than 1% as determined by silver-stained SDS-PAGE gel.

Biological Activity:
The biological activity was determined by measuring the dose dependent mobilization of intracellular calcium (calcium flux) with human neutrophils. Significant calcium mobilization is observed with ≥100ng/mL of recombinant human ENA-78.

Endotoxin:
Less than 0.1 ng/µg (IEU/µg) of rHuENA-78.

Protein content:
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm.
2. Analysis by RP-HPLC, using a calibrated solution of ENA-78 as a Reference Standard.