CATALOG #: 5030

PRODUCT DESCRIPTION:
Recombinant Human soluble 4-1BB Receptor also called Tumor necrosis factor receptor superfamily member 9 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 167 amino acids, having a molecular mass of 17718 Dalton and containing the cysteine rich TNFR-like extracellular domain of 4-1BB Receptor. The rHu4-1BB Receptor is purified by proprietary chromatographic techniques.

SOURCE:
Escherichia Coli.

FORMULATION:
Lyophilized from a concentrated (1mg/ml) solution in water containing no additives.

PURITY:
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(c) Analysis by SDS-PAGE

BIOLOGICAL ACTIVITY:
rHu4-1BBr is fully biologically active when compared to standard. The activity was determined by the inhibition of 4-1BB ligand mediated stimulation of IL-8 production by human PBMC. Results: 90% inibition using 1ug for both 4-1BB ligand and 4-1BB receptor.

STORAGE:
Although stable at room temperature for 3 weeks, it should be stored desiccated below -18°C. Upon reconstitution it should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.

SOLUBILITY:
It is recommended to reconstitute in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.