CATALOG #: 7047

PRODUCT DESCRIPTION:
M-GM-CSF is produced in response to a number of inflammatory mediators by mesenchymal cells present in the hemopoietic environment and at peripheral sites of inflammation. GM-CSF is able to stimulate the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells and can stimulate the formation of eosinophil colonies from fetal liver progenitor cells. GM-CSF can also stimulate some functional activities in mature granulocytes and macrophages. GM-CSF receptors shows significant homologies with other receptors for hematopoietic growth factors , including IL2-beta, IL-3, IL-6, IL-7, EPO and the Prolactin receptors. Recombinant murine GM-CSF produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 125 amino acids and having a molecular mass of 14285.35 Dalton.

SOURCE:
E.coli.

FORMULATION:
Murine GM-CSF was lyophilized after extensive dialysis against 2mM sodium phosphate buffer pH= 7.4.

RECONSTITUTION:
It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mgs/ml, which can be further diluted into other aqueous solutions.

STABILITY:
Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.

BIOLOGICAL ACTIVITY:
Murine GM-CSF is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of the proliferation of murine FDC-P1 cell line is less then 0.2 ng/ml, corresponding to a Specific Activity of 5x10 6IU/mg.

PURITY:
Greater than 95% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.

PROTEIN CONTENT DETERMINED BY:
UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.