CATALOG #: 7046

PRODUCT DESCRIPTION:
FGF is a single-chain polypeptide growth factor that plays a significant role in the process of wound healing and is a potent inducer of angiogenesis. Several different forms of the protein exist ranging from 18-24 kDa in size due to the use of alternative start sites within the fgf-2 gene. It has a 55 percent amino acid residue identity to FGF-1 and has potent heparin-binding activity. The growth factor is an extremely potent inducer of DNA synthesis in a variety of cell types from mesoderm and neuroectoderm lineages. It was originally named basic fibroblast growth factor based upon its chemical properties and to distinguish it from acidic fibroblast growth factor ._Other homologous FGF belonging to the same family are int-2 (FGF-3), FGF-5, FGF-6, K-FGF and KGF (keratinocyte growth factor =FGF-7 ). All factors are products of different genes, some of which are Oncogene products (FGF-3, FGF-4, FGF-5). Recombinant Mouse basic FGF (FGF-2) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 145 amino acids and having a molecular mass of 16320 Dalton.

SOURCE:
E.coli.

FORMULATION:
Lyopohilized from 5 mM NaP, 7.5 + 75 mM NaCl.

RECONSTITUTION:
It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mgs/ml, which can be further diluted into other aqueous solutions.

STABILITY:
Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.

BIOLOGICAL ACTIVITY:
The ED50, calculated by the dose-dependant proliferation of BALB/3T3 cells is <0.5 ng/ml.

PURITY:
Greater than 95% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.

PROTEIN CONTENT DETERMINED BY:
UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.