CATALOG #: 7045

PRODUCT DESCRIPTION:
Recombinant Murine Fibroblast Growth Factor-acidic is one of over 20 members of the FGF family. The fibroblast growth factors play a central role in prenatal development and growth and regeneration of cells during the proliferation and differentiation stages. M-FGF-acidic is non-glycosylated heparin binding protein comprised of 141 amino acid residues with a molecular weight of 15.9 kDa.

SOURCE:
E.coli.

FORMULATION:
Lyophilized at a concentration of 1 mg/ml in 5 mM NaP, 7.5 + 75 mM NaCl.

RECONSTITUTION:
It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 - 0.5 mgs/ml, which can be further diluted into other aqueous solutions.

STABILITY:
Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.

BIOLOGICAL ACTIVITY:
The ED50 as determined by the dose-dependent stimulation of thymidine uptake by 3T3 cells in the presence of heparin is d 0.5 ng/ml corresponding to a specific activity of e 2 x 106 units/mg.

PURITY:
Greater than 95% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.

PROTEIN CONTENT DETERMINED BY:
UV spectroscopy at 280 nm._Analysis by RP-HPLC calibrated against a known standard._Quantitation on SDS-PAGE against a known standard.